Application of HPLC Analysis of Medroxyprogesterone Acetate in Human Plasma

Generally, progestogens, i.e. progesterone in combination with oestradiol esters, are used primarily in the treatment of menstrual irregularities to maintain endangered pregnancies. Their derivatives are orally active medroxyprogesterone acetate including hydroxyprogesterone caproate, chlormadinone acetate, megestrol acetate. Naturally occurring glucocorticoids are often used in medicine such as cortisone, hydrocortisone, prednisone and prednisolone (Fig. 1). Depot-medroxyprogesterone acetate (Depot-MPA) is commercially available and commonly known as a hormonal contraception used by women. It is a synthetic steroid hormone, which mimics natural progesterone, but its contraceptive activity has been shown to be about 30 times higher. Depot-MPA is widely used by intramuscular administration for long-term contraception [1-3].